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Cosa sono gli RF?

Posted on Novembre 24, 2022 By Author

Sommario

  • 1 Cosa sono gli RF?
  • 2 Cosa è la fase Mobile o eluente?
  • 3 Do more polar compounds travel further in normal phase TLC?
  • 4 What is the difference between polar and nonpolar chromatography?
  • 5 What is the stationary phase in TLC?

Cosa sono gli RF?

Il valore Rf è il fattore di ritenzione utilizzato nell’identificazione di composti organici in una miscela. Il valore Rf viene calcolato misurando la distanza relativa percorsa da un particolare composto organico rispetto alla fase mobile. È calcolato in cromatografia su carta e TLC.

Cosa è la fase Mobile o eluente?

L’eluente (o fase mobile) è il vettore che provoca il trasporto delle sostanze chimiche all’interno della colonna. Nella cromatografia liquida l’eluente è il liquido usato come solvente; nella gascromatografia è il gas di trasporto.

Come misurare Rf?

Il valore Rf di un composto è uguale alla distanza percorsa dal composto diviso per la distanza percorsa dal fronte del solvente (entrambi misurati dall’origine).

Leggi anche:   Cosa fare se si ingerisce acido borico?

Do more polar compounds travel further in normal phase TLC?

Never have I seen a normal phase (i.e. silica gel or alox) TLC where a more polar compounds travels further than a less polar compound — and I have used some extremely (considering normal phase TLC) polar eluents in the past. The top-running compounds are always the least polar and the slowest are always the most polar in normal phase TLC.

What is the difference between polar and nonpolar chromatography?

Non-polar compounds move up the plate most rapidly (higher Rf value), whereas polar substances travel up the TLC plate slowly or not at all (lower Rf value). Similarly, how does polarity affect chromatography?

Is ethyl acetate polar or non-polar?

$\\begingroup$ The link seems to have disappeared. Ethyl acetate is polar. Hexane is non-polar. So by using a mixture of the two you can get solutions of varying polarity. Adjusting polarity that way is easier than trying to find 10 different solvents of varying polarity.

Leggi anche:   Chi ha inventato il sistema temperato?

What is the stationary phase in TLC?

TLC uses a stationary phase, usually alumina or silica, that is highly polar (standard) or non-polar (reverse phase), and a mobile phase, some solvent whose polarity you will choose. In 5.301, and in most lab applications, you will use standard phase silica plates.

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